STUDIES ON DNA FROM NORMAL AND SCRAPIE‐AFFECTED MOUSE BRAIN

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Abstract
Abstract— DNA has been extracted from normal and scrapie affected mouse brain fractions, 48 h after the injection of [3H]thymidine precursors. The extracted DNA has been subjected to fractionation on hydroxyapatite columns and CsCl gradients. The specific activity of double stranded nuclear DNA is two to three times higher when extracted from scrapie‐affected brain than from normal brain, but there is no apparent difference in the number of counts associated with double stranded mitochondrial DNA extracted from similar numbers of normal and scrapie affected brains. DNA from the large granule fraction of scrapie affected brain contains a peak of counts, melting off hydroxyapatite columns before the double stranded peak, consistent with the presence in scrapie brain of trace amounts of a small single stranded DNA.