Information Theoretical Approach to Single-Molecule Experimental Design and Interpretation
- 18 July 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in The Journal of Physical Chemistry A
- Vol. 110 (31) , 9743-9757
- https://doi.org/10.1021/jp062192b
Abstract
We use Shannon's definition of information to develop a theory to predict a photon-counting-based single-molecule experiment's ability to measure the desired property. We treat three phenomena that are commonly measured on single molecules: spectral fluctuations of a solvatochromic dye; assignment of the azimuthal dipole angle; determination of a distance by fluorescence resonant energy transfer using Förster's theory. We consider the effect of background and other “imperfections” on the measurement through the decrease in information.Keywords
This publication has 46 references indexed in Scilit:
- Real-Time Dipole Orientational Imaging as a Probe of Ligand−Protein InteractionsThe Journal of Physical Chemistry B, 2005
- Single‐Molecule Fluorescence Spectroscopy of Protein FoldingChemphyschem, 2005
- Single-Molecule Spectroscopy of Conjugated PolymersAccounts of Chemical Research, 2005
- Generalized Langevin Equation with Fractional Gaussian Noise: Subdiffusion within a Single Protein MoleculePhysical Review Letters, 2004
- Single-Molecule Resonance Energy Transfer and Fluorescence Correlation Spectroscopy of Calmodulin in SolutionThe Journal of Physical Chemistry B, 2004
- Structural Dynamics and Processing of Nucleic Acids Revealed by Single-Molecule SpectroscopyBiochemistry, 2004
- Single-molecule fluorescence spectroscopy of biomolecular foldingJournal of Physics: Condensed Matter, 2003
- Measurement of Submicrosecond Intramolecular Contact Formation in Peptides at the Single-Molecule LevelJournal of the American Chemical Society, 2003
- Spectral Dynamics of Individual Molecules in Glasses and CrystalsAccounts of Chemical Research, 1996
- Information Theory and Statistical MechanicsPhysical Review B, 1957