Production of Monoclonal Antibodies to Viruses in the Potyvirus Group: Use in Radioimmunoassay

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Abstract
Mouse hybridomas were developed that secreted antibodies to soybean mosaic (SMV), lettuce mosaic (LMV) viruses and to maize dwarf mosaic (MDMV) virus, strains Ap and B. All hybridomas produced antibodies specific to the homologous virus except for one to LMV, which produced antibodies that reacted at a low level with SMV and MDMV. Monoclonal antibodies against SMV were used in a double-antibody sandwich radioimmunoassay. The assay using only 1 type of monoclonal antibody lacked sensitivity because, presumably, limited epitopes were available. Results obtained using 2 monoclonal antibodies that bound to different epitopes were comparble to those using polyclonal antibodies. A competitive radioimmunoassay, using a single monoclonal antibody, was developed to detect successfully SMV, LMV and MDMV.