RNA‐DNA HYBRIDIZATION STUDIES WITH UNIQUE MOUSE DNA SEQUENCES1

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Abstract
Abstract— Unique mouse DNA sequences (not reassociated at cot 200) were prepared and hybridized with RNA fractions extracted at different temperatures with phenol from brain, liver and plasmocytoma cells. The hybridizations were carried out in liquid media, and the RNA‐DNA hybrids formed were separated on hydroxyapatite columns. The stringency of hybridization was measured by the sensitivity of the hybrids towards nuclease St, and by the Tm's of the RNA‐DNA hybrids.A tissue specificity of RNA transcription has been found. Brain RNA's seemed to be more complex than those of liver. Brain RNA fraction I (primarily of cytoplasmic origin) hybridized to unique DNA sequences more efficiently than RNA fractions II + III (enriched in nuclear messenger RNA's) whereas the reverse was true for liver and plasmocytoma cell RNA.