The resting membrane potential of frog sartorius muscle.

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Abstract
The absolute resting membrane potential of the frog sartorius muscle was determined in Ringer solution at 20.degree. C. Membrane potentials were measured with 0.03, 0.1, 0.3, 1.0 and 3.0 M-KCl electrodes; potentials, including the micro-electrode-tip potentials, were -62.4, -75.7, -83.0, -87.7 and -90.7 mV, respectively. Membrane potentials were corrected for the liquid junction potentials between the Ringer and KCl solutions, and the myoplasm and the KCl solutions of the micro-electrodes, using the Henderson''s liquid junction equation. The cation (U) and anion (V) conductances of the myoplasm were adjusted to give a constant membrane potential after correction for liquid junction potentials. The final values for U and V were 5.6 and 1.3 mmho/cm, respectively, resulting in a membrane potential of -93.0 mV. The correction for the liquid junction potential was -2.6 mV when a 3 M-KCl micro-electrode was used.