Phase Transition and Phase Segregation in a Single Double-Stranded DNA Molecule
- 2 September 1996
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review Letters
- Vol. 77 (10) , 2133-2136
- https://doi.org/10.1103/physrevlett.77.2133
Abstract
From the single-molecule observation of a long DNA chain, T4 DNA (166 kbp), by fluorescence microscopy, we found that an individual chain exhibits a first-order phase transition between an elongated coil and a compact globule with a change in the concentration of alcohol added to the aqueous environment. In the globule phase near the transition point, a metastable state is observed in which the globular and coil states coexist within a single DNA chain, i.e., phase separation is induced along a single chain.Keywords
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