PATTERN OF ENDOGENOUS LECTINS IN A HUMAN EPITHELIAL TUMOR

  • 1 January 1985
    • journal article
    • research article
    • Vol. 45  (1) , 253-257
Abstract
Salt and detergent extracts of a malignant epithelial tumor, obtained by extraction of acetone powder, were fractionated on different sets of Sepharose columns covalently derivatized with lactose, asialofetuin, melibiose, mannan, fucose and heparin. Successive elution by chelating reagent and specific sugar resulted in isolation of different Ca2+-dependent and Ca2+independent endogenous carbohydrate-binding proteins, as analyzed by gel electrophoresis. It appears from the analysis that certain bands represent newly identified proteins capable of binding to lactose (at MW 64,000), melibiose (at MW 28,000) and fucose (at MW 62,000 and 70,000). Other carbohydrate-binding proteins isolated from this human tumor were identified in normal, especially embryonic, tissues of different nonhuman vertebrates. The carbohydrate-binding proteins were assayable as agglutinin with rabbit erythrocytes and showed no detectable enzymatic activity. They can thus be defined as lectins. The presence of a complex pattern of endogenous lectins and their biochemical characteristics may contribute to an understanding of intercellular interaction during the complex process of metastatic spread and may furthermore allow a new tool for diagnosis and a lectin-based therapy.

This publication has 0 references indexed in Scilit: