Properties of an Oxidative Phosphorylation System Reconstituted from Coupling Factors in Micrococcus lysodeikticus*

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Abstract
A system for oxidative phosphorylation was reconstituted from three components of Micrococcus lysodeikticus: membrane fragments, coupling factor cF and coupling factor Ft, or from membrane fragments and either one of these coupling factor. ATPase [EC 3.6.1.3] activity which was sensitive to the cation translocating uncoupler, gramicidin A was also reconstituted with this system. It was suggested that the reconstitution of this gramicidin sensitive ATPase indicated the partial or total reconstitution of energy transfer starting from ATP. The effects of other antibiotics affecting ion transport across the inner mitochondrial membrane were also tested on the reconstituted phosphorylation and ATPase systems. The effects of trypsin [EC 3.4.4.4] on the isolated coupling factors and on the reconstituted system were examined. Only the isolated factors were highly susceptible to trypsin, whereas the reconstituted system was resistant in the presence of divalent cations. The results were compared with those on reconstituted mitochondrial and chloroplast energy transfer systems, and with other ATPase systems of the bacterial membrane.

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