Detection of Aeromonas hydrophila in food with an enzyme‐linked immunosorbent assay
- 1 February 1993
- journal article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 74 (2) , 149-154
- https://doi.org/10.1111/j.1365-2672.1993.tb03008.x
Abstract
A microtitration plate, antibody capture, enzyme‐linked immunosorbent assay was developed for the detection of Aeromonas hydrophila serotype O : 11 (highly virulent strains). The assay utilizes a detector antibody which shows no cross‐reactions with Aeromonas strains other than serotype O : 11 or non‐Aeromonas competing organisms. The detector antibody is mixed with the sample and incubated for 1 h, microcentrifuged and the supernatant fluid (unadsorbed antibody) titred in a microtitre plate coated with A. hydrophila cells from serotype O : 11. All the A. hydrophila strains from serotype O : 11 tested reacted strongly with the detector antibody. Also by culturing and performing the immunoassay with the detector antibody we established and quantified the presence of A. hydrophila O : 11 in different foods.Keywords
This publication has 18 references indexed in Scilit:
- Isolation and characterization of bacteriophage PM3 fromAeromonas hydrophilathe bacterial receptor for which is the monopolar flagellumFEMS Microbiology Letters, 1990
- Isolation and characterization of bacteriophage PM2 fromAeromonas hydrophilaFEMS Microbiology Letters, 1990
- Surface protein composition of Aeromonas hydrophila strains virulent for fish: identification of a surface array proteinJournal of Bacteriology, 1988
- Importance of Aeromonas sobria in Aeromonas BacteremiaThe Journal of Infectious Diseases, 1987
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951