MECHANISM FOR EXCLUSION OF 5-FLUOROURACIL FROM DNA

Google Scholar
  • 1 January 1980
    • journal article
    • research article
    • Vol. 40  (4) , 998-1001
Abstract
5-Fluorodeoxyuridine 5''-triphosphate [derived from the metabolism of the anticancer chemotherapeutic 5-fluorouracil in Escherichia coli] is hydrolyzed by the enzyme deoxyuridine triphosphate diphosphohydrolase (EC 3.6.1.23). Uracil-DNA glycosylase removes 5-fluorouracil (FUra) from FUra-containing DNA similar to its removal of uracil from uracil-containing DNA. The absence of FUra in DNA following exposure of cells to FUra can be explained by the activities of these 2 enzymes.