Production of First Component of Complement by Corneal Fibroblasts in Tissue Culture
- 1 March 1982
- journal article
- research article
- Published by American Medical Association (AMA) in Archives of Ophthalmology (1950)
- Vol. 100 (3) , 478-480
- https://doi.org/10.1001/archopht.1982.01030030480022
Abstract
• Corneal fibroblasts were studied to determine if they have the ability to synthesize and secrete complement components in tissue culture. Culture media were assayed for functional complement activity of C1, C4, C2, C3, C5, C6, and C7 with the use of 50% hemolysis of sensitized sheep RBCs. Only C1 showed a progressive increase in hemolytic activity at days 3, 5, 7, 9, and 11 of tissue culture. This increase in hemolytic C1 could be reversibly inhibited by cycloheximide, an inhibitor of protein synthesis. Corneal fibroblasts may be a potential source of C1 in the cornea.This publication has 5 references indexed in Scilit:
- Distribution of Hemolytic Complement in the Normal CorneaArchives of Ophthalmology (1950), 1981
- Hemolytic Complement Activity in Normal Human Donor CorneasArchives of Ophthalmology (1950), 1980
- Alternate and Classical Pathway Components of Complement in the Normal CorneaArchives of Ophthalmology (1950), 1980
- C3 component of complement secreted by established cell linesCell, 1978
- Biosynthesis of the first component of complement by human fibroblastsBiochemical Journal, 1977