Specificity of DC‐SIGN for mannose‐ and fucose‐containing glycans
Open Access
- 16 October 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (26) , 6123-6131
- https://doi.org/10.1016/j.febslet.2006.10.009
Abstract
The dendritic cell specific C‐type lectin dendritic cell specific ICAM‐3 grabbing non‐integrin (DC‐SIGN) binds to “self” glycan ligands found on human cells and to “foreign” glycans of bacterial or parasitic pathogens. Here, we investigated the binding properties of DC‐SIGN to a large array of potential ligands in a glycan array format. Our data indicate that DC‐SIGN binds with K d < 2 μM to a neoglycoconjugate in which Galβ1‐4(Fucα1‐3)GlcNAc (Lex) trisaccharides are expressed multivalently. A lower selective binding was observed to oligomannose‐type N‐glycans, diantennary N‐glycans expressing Lex and GalNAcβ1‐4(Fucα1‐3)GlcNAc (LacdiNAc‐fucose), whereas no binding was observed to N‐glycans expressing core‐fucose linked either α1‐6 or α1‐3 to the Asn‐linked GlcNAc of N‐glycans. These results demonstrate that DC‐SIGN is selective in its recognition of specific types of fucosylated glycans and subsets of oligomannose‐ and complex‐type N‐glycans.Keywords
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