Down-modulation of c-kitmRNA and protein expression by erythroid differentiation factor/activin A
Open Access
- 23 October 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 374 (1) , 69-71
- https://doi.org/10.1016/0014-5793(95)01078-s
Abstract
We examined the effects of erythroid differentiation factor (EDF)/activin A on the expression of c‐kit mRNA and protein in murine erythroleukemia (MEL) cells. EDF/activin A induced MEL cells to benzidine positive cells. Northern blot analysis showed that the c‐kit mRNA expression was reduced synchronously with increase of β‐globin and uroporphyrinogen decarboxylase gene expression during EDF/activin A induced erythroid differentiation. Scatchard analysis indicated that the cell surface receptor number was reduced without change of affinity during differentiation. Our results suggest that EDF/activin A may act as a natural regulator of erythropoiesis with modulation of c‐kit gene expression.Keywords
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