Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction
Open Access
- 1 April 1999
- journal article
- Published by FapUNIFESP (SciELO) in Revista de Microbiologia
- Vol. 30 (2) , 144-148
- https://doi.org/10.1590/s0001-37141999000200011
Abstract
Mycobacterium avium complex (MAC) species cannot be discriminated by the usual methods of biochemical identification of mycobacteria. This study showed that amplification by PCR of DT1 and DT6, two single copy sequences identified in the genome of M. avium serotype 2, the insertion sequence IS1245, found to be consistently present in M. avium strains and the heat-shock protein gene hsp65, followed by restriction polymorphism analysis, are rapid and accurate tests for the differentiation of the species M. avium, M. intracellulare, and M. scrofulaceum.Keywords
This publication has 15 references indexed in Scilit:
- Molecular characterization of Mycobacterium avium complex isolates giving discordant results in AccuProbe tests by PCR-restriction enzyme analysis, 16S rRNA gene sequencing, and DT1-DT6 PCRJournal of Clinical Microbiology, 1997
- Genotypic characterization of Mycobacterium avium strains recovered from animals and their comparison to human strainsApplied and Environmental Microbiology, 1995
- Epidemiology of Mycobacterium avium infections in the pre- and post-HIV eraResearch in Microbiology, 1994
- Isolation of Mycobacterium avium Complex from Bone Marrow Aspirates of AIDS Patients in BrazilThe Journal of Infectious Diseases, 1993
- The Mycobacterium avium complexClinical Microbiology Reviews, 1993
- Isolation of specific DNA fragments of Mycobacterium avium and their possible use in diagnosisJournal of Clinical Microbiology, 1993
- Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysisJournal of Clinical Microbiology, 1993
- Towards a Phylogeny and Definition of Species at the Molecular Level within the Genus MycobacteriumInternational Journal of Systematic and Evolutionary Microbiology, 1990
- Differentiation of Mycobacterium species by direct sequencing of amplified DNAJournal of General Microbiology, 1990
- Identification of various serovar strains of Mycobacterium avium complex by using DNA probes specific for Mycobacterium avium and Mycobacterium intracellulareJournal of Clinical Microbiology, 1990