9-Anthryldiazomethane-HPLC method for detection of prostaglandins and thromboxane : An application to the measurement of the products of stimulated rabbit platelets.
- 31 December 1985
- journal article
- letter
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 34 (8) , 3526-3529
- https://doi.org/10.1248/cpb.34.3526
Abstract
The cyclooxygenase products of rabbit platelets stimulated with arachidonic acid were measured by HPLC. Prostaglandins and thromboxane were extracted, derivatized with 9-anthryldiazomethane, and then loaded on an ODS-column. The fluorescence of the eluate was monitored. Thromboxane B2 was a major product, which returned to the control level in the presence of indomethacin or the thromboxane synthetase inhibitor OKY-046. Pretreatment with OKY-046 shifted the cyclooxygenase products to increased levels of PGE2, PGD2 and PGF2.alpha.This publication has 1 reference indexed in Scilit:
- Prostaglandins and human platelet aggregationBiochemical Pharmacology, 1985