Sub‐picosecond measurements of primary electron transfer inRhodopseudomonas viridisreaction centers using near‐infrared excitation
- 18 August 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 204 (2) , 368-372
- https://doi.org/10.1016/0014-5793(86)80845-6
Abstract
Rate constants for the primary photoinduced electron transfer reactions within the reaction center protein of the photosynthetic bacteriumRhodopseudomonas viridishave been measured using transient optical absorption spectroscopy following excitation of the primary donor P960 directly with 950 nm laser flashes. The time resolution of the experiments was 0.45 ps. The reduction of BPheobas indicated by absorption changes at 543 nm exhibits monophasic kinetics with a 1.7 × 1011s1̄rate constant following direct excitation of P960. Spectral changes at 805 and 835 nm due to perturbations of the intermediary BChlboccur synchronously with those at 543 nm. There is no evidence for formation of BChlb−as a distinct chemical intermediate preceding the reduction of BPheob.Keywords
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