Temperature and Age as Factors in Inducing Insemination of Mosquitoes (Diptera: Culicidae)1

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Abstract
Many species of mosquitoes are unsuited to life in a laboratory because they fail to copulate in cages. This situation may be largely eliminated by the technique in current use for manually inducing insemination as outlined. The pre-mating age of the males and the temperature at which they are held affect the ability of males to transfer sperm. Mating males 14 days old with females at any age between 3 and 14 days resulted in a high order of insemination of a wild strain of Aedes vexans (Meigen). Pre-mating engorgement of females and a humidity of the air of 85+% RH enhanced the degree of insemination.

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