A Rapid Automated Latex Screen for Tetanus Toxoid Antibodies

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Abstract
This report describes the sensitization of latex particles and the use of this reagent on the Autotape system to select [human] blood donors containing .gtoreq. 4 IU/ml of tetanus toxoid antibody. Plasma containing tetanus toxoid antibodies to processed to provide immunoglobulin for passive protection against tetanus. The techniques used for screening include immunoelectrophoresis, tetanus toxoid sensitized latex particles, hemagglutination and automated hemagglutination. The technique used by the Sheffield Regional Transfusion Center (until recently) was immunoelectrophoresis, selecting donors with 4 or more IU/ml. The Autotape system, previously described for brucellosis and syphilis testing, provides a system for automated slide testing. A 5-min tetanus toxoid coated latex test was developed to select suitable donations for the production of specific immunoglobulin.