Estimation of the average section thickness in ribbons of ultra‐thin sections

Publisher Website
Google Scholar
Abstract
SUMMARY: A simple procedure for estimating the depth to which a ribbon of ultra‐thin sections cuts into a block is described. The average section increment is obtained by dividing the depth by the number of sections in the ribbon. Comparison with measurements of individual sections made using interference microscopy suggests: (a) that loss of resin brought about by the cutting process is insignificant, i.e. that the average section increment equals the average section thickness, and (b) that there can be considerable variation in section thickness along a ribbon. Provided that such variation is taken into account, the method based on measurements of the block face is simple, avoids time‐consuming alternative procedures, and is appropriate when seeking to determine the depth dimension to be used in making three‐dimensional reconstructions from long sequences of sections.

This publication has 3 references indexed in Scilit: