Progesterone in Blood Plasma of the Ovulating Hen

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Abstract
Plasma samples were taken from White Leghorn hens in which the interval between successive ovulations was ca. 26.5 hrs. Timing of withdrawal of blood samples was referred to 2d ovulation of the clutch but these may be considered as having been taken between two consecutive ovulations, ca. 6.5 hrs. elapsing between withdrawal of the first and the last of 10 samples. Each plasma sample was prepd. from 0.95 ml. of blood plus ca. 0.05 ml. of Na citrate, centrifuged and frozen until tested. The samples were tested by a recent method (Hooker and Forbes, Endocrinology 41:158-169. 1947). A positive reaction indicates a progesterone concn. greater than 0.33 micrograms per ml. of plasma a negative reaction signifies a concn. less than that value. The samples gave 8 positive and 2 negative reactions. This is evidence for the presence of progesterone in the blood of the ovulating hen. The negative reactions were given by samples taken at or near release of ovulation-inducing hormone. This is evidence for the ovarian or follicular origin of progesterone.

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