Detection and enzymatic deglycosylation of a glycosylated variant of prolactin in human plasma
- 23 February 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 212 (2) , 220-224
- https://doi.org/10.1016/0014-5793(87)81348-0
Abstract
Immunoperoxidase electrophoresis was applied to the plasma of a patient showing a high level of prolactin (PRL) secreted by a pituitary adenoma. Two PRL monomers were detected with an anti‐hPRL antiserum: a major 22 kDa form and a minor 25 kDa form. Concanavalin A‐Sepharose 4B chromatography revealed that the 25 kDa form was a glycosylated variant of PRL. Incubation of this variant with endoglycosidase F led to its transformation into the 22 kDa form.Keywords
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