Effects of Carbon and Nitrogen Sources on the Levels of Several NADP- and NAD-Linked Dehydrogenase Activities of Hydrocarbon-utilizableCandidaYeasts

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Abstract
The variation of activities of several NADP-linked and NAD-linked dehydrogenases were studied during the aerobic growth of two species of hydrocarbon-utilizable Candida yeasts on different carbon and nitrogen sources. The level of NADP-linked isocitrate dehydrogenase in C. tropicalis and C. lipolytica growing on acetate was significantly higher than that in the yeasts growing on glucose. The glucose-grown cells of C. tropicalis showed a high activity of glucose-6-phosphate dehydrogenase as compared with the acetate-grown cells, while the enzyme level in C. lipolytica was low regardless of carbon sources used. The cells of both yeasts growing on n-alkane and oleic acid contained relatively low activity of NADP-linked isocitrate dehydrogenase. Presence of ion in the acetate medium increased the level of NADP-linked isocitrate dehydrogenase activity. These results suggest that different types of NADPH-generating systems operate alternatively in these yeasts depending upon carbon and nitrogen sources.

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