Subcellular Distribution of Succinate‐Semialdehyde Dehydrogenase in Rat Brain

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Abstract
The report details the subcellular localization of succinate semialdehyde dehydrogenase in rat brain. The localization of this enzyme, which is important in the catabolism of 4‐aminobutyrate and brain intermediary energy metabolism, is compared with glutamic acid decarboxylase and lactate dehydrogenase. A unique significant over‐recovery of succinic semialdehyde dehydrogenase activity was observed in sucrose gradient subfractions, an over‐recovery which could be eliminated by repeated freeze‐thaws or Triton treatment of fractions. Triton treatment also revealed a significant amount of this activity in the synaptosomal fraction not apparent in the standard procedure, but the major enrichment of activity was found in the mitochondrial fraction. Our results indicate that a striking separation exists in the cellular localization of the synthetic reaction for 4‐aminobutyrate in brain and the catabolic system represented by succinate semialdehyde dehydrogenase.