Determination of Triiodothyronine in Serum by Enzyme- and Radioimmunoassay. A Comparative Study

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Abstract
An evaluation of a heterogeneous enzyme immunoassay for determination of triiodothyronine [T3] in human serum (Enzymum-Test T3, Boehringer Mannheim) is presented. The enzyme immunoassay was compared with the laboratory routine radioimmunoassay [RIA]. The precision of both assays was satisfactory at T3 concentrations between 1.0 and 8.0 nmol/l (coefficients of variation from day to day .ltoreq. 10%). Average recoveries of T3 in pool sera ranged with the Enzymun-Test T3 from 961-104% and with the RIA from 88-111%. A comparison of the results obtained by Enzymun-Test T3 and the RIA in a series of 103 patients showed a good correlation between both methods. L-thyroxine did not cause a relevant cross-reaction in the enzyme immunoassay. About 20 unknown samples can be analyzed in triplicate by Enzymun-Test T3 within 260 min.

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