DNA POLYMERASE ACTIVITY DETECTED IN THE NUCLEI OF DRY BARLEY SEED EMBRYOS
- 1 January 1976
- journal article
- research article
- Published by Genetics Society of Japan in The Japanese Journal of Genetics
- Vol. 51 (1) , 59-60
- https://doi.org/10.1266/jjg.51.59
Abstract
The activity of DNA polymerase, determined by tritiated deoxynucleotide incorporation into DNA per .mu.g protein, in crude extracts decreased with increasing amount of the protein. This result suggests that nucleolytic enzymes in the crude extracts interfered in DNA polymerization. In order to inhibit such nucleolytic activities, various amount of NaF was added to the reaction mixture for DNA polymerase assay. Activity of DNA polymerase was the highest when about 15 mM NaF was added to the reaction mixture. The pH optimum of the DNA polymerase reaction in the crude extract was 9.0.This publication has 1 reference indexed in Scilit:
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951