Synthetic Analogues of Residues 1-34 of Human Parathyroid Hormone: Influence of Residue Number 1 on Biological Potencyin Vitro
- 1 January 1975
- journal article
- Published by Taylor & Francis in Endocrine Research Communications
- Vol. 2 (8) , 561-570
- https://doi.org/10.3109/07435807509050679
Abstract
A biologically active tetratriacontapeptide of human parathyroid hormone, hPTH(1-34), has been synthesized together with a series of structural analogues involving changes at the amino-terminal residue. Acetylation of the terminal amino group results in a marked reduction in the biological potency as measured in the in vitro rat renal adenylyl cyclase assay. Deletion of the terminal amino group results in a loss of biological activity. Substitution of the amino-terminal serine residue with glycine gives a lowered potency whereas substitution with alanine results in a 2-5-fold increase in biological activity in the in vitro assay. The results are compared with the findings previously reported for a series of amino-terminal analogues of the bovine PTH 1-34 peptide.Keywords
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