NON‐CONTACT SPECULAR MICROSCOPY OF HUMAN CORNEAL ENDOTHELIUM

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Abstract
The non-contact specular microscope enables a large area of the corneal endothelium to be examined and photographed without any risk of traumatizing the anterior surface of cornea. This non-contact technique is described in detail. SD of the relative difference between 2 independent estimates of central endothelial density from 24 eyes was 3.7%. SD of the relative difference between counts from left and right eyes from 57 subjects was 4.7%. A significant negative correlation between age and endothelial cell density was found (r = -0.59, P < 0.001, n = 76). The interindividual variation in cell densities was larger in the old age group, indicating that cell loss during aging varies among different individuals.

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