In vitro transcription initiation from three different Escherichia coli promoters
- 1 April 1985
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 148 (2) , 293-298
- https://doi.org/10.1111/j.1432-1033.1985.tb08838.x
Abstract
Transcription initiation from .beta.-lactamase, tetracycline resistance and RNA1 promoters, present in plasmid pAT153, were studied employing the abortive initiation technique. Assays appear to be promoter-specific with supercoiled and linear templates. Supercoiling enhances the isomerization rate constant of the open RNA-polymerase-promoter complex formation. Results agree with the in vivo behavior of the corresponding promoters, and show the effect of supercoiling on transcription initiation.This publication has 28 references indexed in Scilit:
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