ISOLATION OF 6 MONOCLONAL ALLOANTIBODIES AGAINST RAT HISTOCOMPATIBILITY ANTIGENS - CLONAL COMPETITION
- 1 January 1980
- journal article
- research article
- Vol. 41 (1) , 131-141
Abstract
The isolation of 6 clones and some variant derivatives of rat .times. mouse hybrid myelomas [rat spleen cell mouse myeloma P3-X63-Ag8 cell hybridoma] secreting alloantibody against antigens of the rat major histocompatibility complex is described. Very large numbers of active hybrids were obtained but many were lost early in the post-fusion period; evidence is presented for rapid selective processes operating in uncloned complex hybrid cultures. The secretion of specific Ig chains probably is as stable a function in rat .times. mouse hybrid myelomas as in mouse .times. mouse hybrid myelomas.This publication has 12 references indexed in Scilit:
- Monoclonal Antibodies as Tools to Analyze the Serological and Genetic Complexities of Major Transplantation AntigensImmunological Reviews, 1979
- Fine Specificity Analysis with Monoclonal Antibodies of Antigens Controlled by the Major Histocompatibility Complex and by the Qa/TL Region in MiceImmunological Reviews, 1979
- Reactivity of Monoclonal Antibodies Specific for H-2 Antigenic Determinants with Cells of Wild MiceImmunological Reviews, 1979
- Anti-Viral and Anti-Tumor Antibodies Produced by Somatic Cell HybridsPublished by Springer Nature ,1979
- Monoclonal Antibodies against Murine Cell Surface Antigens: Anti-H-2, Anti-Ia and Anti-T Cell AntibodiesPublished by Springer Nature ,1979
- Selective Suppression of Reactivity to Rat Histocompatibility Antigens by Hybridoma AntibodiesPublished by Springer Nature ,1979
- Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouseNature, 1978
- Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: Differentiation antigens of rat lymphocytesCell, 1977
- Antibodies to major histocompatibility antigens produced by hybrid cell linesNature, 1977
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975