High-performance Liquid Chromatography/Tandem Mass Spectrometry Identification of Salmon Calcitonin Degradation Products in Aqueous Solution Preparations
- 1 January 1996
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 10 (2) , 151-156
- https://doi.org/10.1002/(sici)1097-0231(19960131)10:2<151::aid-rcm434>3.0.co;2-c
Abstract
Degradation impurities have been investigated, by means of high‐performance liquid chromatography (HPLC)/mass spectrometry, in commercial ampoules of salmon calcitonin, aqueous solution, stored for up to 2 years. Oxidation derivatives of the cysteine residues in positions 1 and 7, with cleavage of the disulfide bridge, were observed in all samples. Using our reversed‐phase separation, these oxidation derivatives coeluted and presented an HPLC behavior identical to the derivative of salmon calcitonin with the disulfide bridge reduced. In a previous study this last compound was tentatively identified, without confirmation by mass spectrometry, as a major degradation product of salmon calcitonin in aqueous solution.Keywords
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