UV-Dependent Quinolone-Induced Human Erythrocyte Membrane Lipid Peroxidation: Studies on the Phototoxicity of Y-26611, a New Quinolone Derivative

Publisher Website
Google Scholar
Abstract
Quinolone antibacterial drugs are widely used as oral therapeutic agents. However, in some patients they cause ultraviolet (UV)‐dependent dermatitis. Using lipid peroxidation as an index of phototoxicity, we studied the effects of a new quinolone derivative, Y‐26611 together with ofloxacin, sparofloxicin and lomefloxacin on washed human erythrocyte suspensions. Irradiation of erythrocytes with UV‐A or UV‐B for 60 min. in the presence of Y‐26611 (30‐600 μg/ml) strong dose dependent lipid peroxidation, up to 17.01 nmoles/ml was induced. Under identical conditions, lipid peroxidation induced by up to 600 μg/ml ofloxacin, sparofloxacin or lomefloxacin were 0.94, 3.36 and 2.98 nmoles/ml respectively. The lipid peroxidation was entirely dependent on both UV as well as the drug. The lipid peroxidation responses to drug + UV could completely be inhibited by sodium azide (hydroxyl radical, HO‐ and singlet oxygen, 1O2 scavenger) or by phenyl N‐tert‐butylnitrone (PBN, HO and superoxide anion radical, O2− scavenger). It is likely that reactive oxygen species generated by interaction between UV‐sensitized drug molecules and oxygen molecules mediate erythrocyte membrane lipid peroxidation. The method used in this study is rapid and convenient for screening drugs for UV‐dependent cytoloxicity.