SEROLOGICALLY H‐Y ANTIGEN‐NEGATIVE XO MICE

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Abstract
SUMMARY: In a series of six independent experiments organ homogenates of 35 mice of the XX, XO or XY sex chromosome constitutions were absorbed using three different anti‐H‐Y antisera raised in inbred female LEW rats. Residual activities of absorbed antisera were tested in the Raji cell, complement‐dependent, cytotoxicity test. Homogenates of various tissues, including the gonads, of XX and XO females were equally unable to absorb H‐Y antibodies, indicating that tissues of these mice do not carry the H‐Y antigen. In contrast, XY male homogenates fully absorbed H‐Y antibodies of antisera at concentrations of ½ to 1/4. We discuss our findings with special attention to the problem of the existence of one or more H‐Y antigens and, to the genetic regultion of the expression of this antigen.