Hd6 , a rice quantitative trait locus involved in photoperiod sensitivity, encodes the α subunit of protein kinase CK2
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- 19 June 2001
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 98 (14) , 7922-7927
- https://doi.org/10.1073/pnas.111136798
Abstract
Hd6 is a quantitative trait locus involved in rice photoperiod sensitivity. It was detected in backcross progeny derived from a cross between the japonica variety Nipponbare and the indica variety Kasalath. To isolate a gene at Hd6 , we used a large segregating population for the high-resolution and fine-scale mapping of Hd6 and constructed genomic clone contigs around the Hd6 region. Linkage analysis with P1-derived artificial chromosome clone-derived DNA markers delimited Hd6 to a 26.4-kb genomic region. We identified a gene encoding the α subunit of protein kinase CK2 (CK2α) in this region. The Nipponbare allele of CK2 α contains a premature stop codon, and the resulting truncated product is undoubtedly nonfunctional. Genetic complementation analysis revealed that the Kasalath allele of CK2 α increases days-to-heading. Map-based cloning with advanced backcross progeny enabled us to identify a gene underlying a quantitative trait locus even though it exhibited a relatively small effect on the phenotype.Keywords
This publication has 28 references indexed in Scilit:
- Hd1, a Major Photoperiod Sensitivity Quantitative Trait Locus in Rice, Is Closely Related to the Arabidopsis Flowering Time Gene CONSTANSPlant Cell, 2000
- fw2.2 : A Quantitative Trait Locus Key to the Evolution of Tomato Fruit SizeScience, 2000
- Antisense Expression of the CK2 α-Subunit Gene in Arabidopsis. Effects on Light-Regulated Gene Expression and Plant Growth1Plant Physiology, 1999
- Light-Regulated TranscriptionAnnual Review of Plant Biology, 1995
- Reconstitution of Arabidopsis casein kinase II from recombinant subunits and phosphorylation of transcription factor GBF1.Plant Cell, 1995
- A 300 kilobase interval genetic map of rice including 883 expressed sequencesNature Genetics, 1994
- Selective amplification of cDNA sequence from total RNA by cassette-ligation mediated polymerase chain reaction (PCR): Application to sequencing 6·5 kb genome segment of hantavirus strain B-1Molecular and Cellular Probes, 1992
- DNA binding activity of the Arabidopsis G-box binding factor GBF1 is stimulated by phosphorylation by casein kinase II from broccoli.Plant Cell, 1992
- Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphismsNature, 1988
- The Protein Kinase Family: Conserved Features and Deduced Phylogeny of the Catalytic DomainsScience, 1988