An effective chemical mutagenesis procedure for Petunia hybrida cell suspension cultures

1 January 1979

journal article
research article
Published by Springer Nature in Theoretical and Applied Genetics

Vol. 55 (3-4) , 101-106
https://doi.org/10.1007/bf00295434

Abstract

By using mercury(II)-chloride (HgCl₂) and Dl-6-fluorotryptophan (6FT) as positive selection conditions we were able to show that N-methyl-N′-nitro-N-nitrosoguanidine (NG) is an effective mutagen for Petunia hybrida suspension cells. A number of the 205 calli resistant to HgCl₂ and 17 calli resistant to 6FT were isolated. The highest mutation frequency was 1.0 x 10⁻⁵ and 2.0 x 10⁻⁶ for HgCl₂ and 6FT, respectively. A preliminary characterization of the mutants is presented. A significant increase in the number of drug-resistant calli was only obtained at NG-concentrations (5–40 μg/ml) that had no observable effect on the survival of the mutagenized cultures.

Keywords

This publication has 10 references indexed in Scilit:

Isolation of Biochemical Mutants of Cultured Plant Cells
Published by Elsevier ,1977
Selection and Characterization of Biochemical Mutants
Proceedings in Life Sciences, 1977
Selection and characterization of cultured carrot and tobacco cells resistant to lysine, methionine, and proline analogs
Canadian Journal of Botany, 1976
Experimental Production of Pollen Haploids and their Uses
Zeitschrift für Pflanzenphysiologie, 1975
Isolation of variant carrot cell lines with altered pigmentation
Phytochemistry, 1974
Anthranilate synthetase from 5-methyltryptophan-susceptible and -resistant cultured Daucus carota cells
Biochimica et Biophysica Acta (BBA) - General Subjects, 1972
The Use of Fluorescein Diacetate and Phenosafranine for Determining Viability of Cultured Plant Cells
Stain Technology, 1972
A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
Physiologia Plantarum, 1962