Improved Cell Growth in Tobacco Suspension Cultures Expressing Vitreoscilla Hemoglobin
- 1 January 2002
- journal article
- research article
- Published by Wiley in Biotechnology Progress
- Vol. 18 (2) , 229-233
- https://doi.org/10.1021/bp010159v
Abstract
Expression of the gene encoding bacterial hemoglobin (VHb) from Vitreoscilla has been previously used to improve recombinant cell growth and enhance product formation under microaerobic conditions, a common phenomenon in large-scale cultivations of bacteria. This technology has now been applied to tobacco suspension cultures. Tobacco suspension cultures have been generated from VHb-expressing tobacco plants. Cell cultures were capable of producing an active hemoglobin. When grown in shake flasks, the cells did not show any lag-phase and exhibited improved cell growth, compared to controls carrying the parental plasmid.Keywords
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