Identification of carbohydrate-binding proteins from mouse and human fibroblasts

Publisher Website
Google Scholar
Abstract
Three carbohydrate-binding proteins (MW 35,000, 16,000 and 13,500) were isolated from extracts of mouse 3T3 fibroblasts by affinity chromatography on polyacrylamide beads to which was covalently bound the ligand 6-aminohexyl 4-.beta.-D-galactosyl-2-acetamido-2-deoxy-.beta.-D-glucopyranoside. None of these proteins bind to polyacrylamide beads coupled with either 6-aminohexanol or 6-aminohexyl .beta.-D-galactopyranoside. Therefore they appear to be carbohydrate-binding proteins specific for galactose-terminated glycoconjugates. A carbohydrate-binding protein was also purified from extracts of human foreskin fibroblasts. This protein (MW 35,000) may represent the human counterpart of the mouse protein of similar MW and binding properties.