A Role for NF-κB Binding Motifs in the Differential Induction of Chemokine Gene Expression in Human Corneal Epithelial Cells

1 July 2004

journal article
Published by Association for Research in Vision and Ophthalmology (ARVO) in Investigative Opthalmology & Visual Science

Vol. 45 (7) , 2299-2305
https://doi.org/10.1167/iovs.03-0367

Abstract

Purpose. The interleukin (IL)-8 promoter possesses a NF-κB–binding site with affinity to p50p65 and p65p65 complexes while the monocyte chemoattractant protein (MCP)-1 promoter’s NF-κB–binding site has exclusive affinity to p50p65 heterodimers. The purpose of this study was to determine whether the two NF-κB sites play a role in the capacity of tumor necrosis factor (TNF)-α–stimulated human corneal epithelial cells (HCECs) to produce nanogram amounts of IL-8 in the absence of MCP-1 synthesis.

Keywords

CHEMOKINE
WILD TYPE
BINDING PROTEIN
CHROMATIN IMMUNOPRECIPITATION
SITE DIRECTED MUTAGENESIS
REPORTER GENE
INTERLEUKIN 8
TRANSCRIPTION FACTOR
TUMOR NECROSIS FACTOR
BINDING SITES
EPITHELIAL CELL
COMPLEX
GENE EXPRESSION
BINDING SITE
OPHTHALMOLOGY
CHROMATIN
TUMOR NECROSIS FACTORS
VISUAL SYSTEM

This publication has 0 references indexed in Scilit: