IRRADIATION OF INDUSTRIAL ENZYME PREPARATIONS II. CHARACTERIZATION OF FUNGAL PECTINASE BY THIN-LAYER ISOELECTRIC FOCUSING AND GEL FILTRATION
- 1 January 1978
- journal article
- Published by Hindawi Limited in Journal of Food Biochemistry
- Vol. 2 (1) , 71-85
- https://doi.org/10.1111/j.1745-4514.1978.tb00604.x
Abstract
Industrial dry fungal pectinase from A. niger was irradiated with doses (up to 1 Mrad) of 60 Co-γrays effective in reducing microbial contamination. The pectinase was characterized by thin-layer isoelectric focusing and gel filtration in order to detect possible radiation-induced structural alterations. Thin-layer isoelectric focusing revealed at least fifteen multiple forms with pectin-depolymerizing activity, with isoelectric points in the range pH 4.5–7. Heterogeneity of pectinesterase was also demonstrated, the main band occurring around pH 4. By thin-layer gel filtration the molecular weight of the pectin-depolymerase was estimated as being about 36,000, and that of pectinesterase as about 33,000. Radiation-induced changes of the charge properties or molecular size of the irradiated pectinase preparation were not observed. The feasibility of using ionizing radiation for the reduction of microbial contamination of industrial enzyme preparations looks promising.This publication has 21 references indexed in Scilit:
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