Biochemical Studies on Ricin Part X Effect of the Two Constituent Polypeptide Chains of Ricin D toward Mouse Sarcoma Ascite Tumor Cells

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Abstract
The effects of isolated ricin chains on the growth of sarcoma 180 ascites (SA) cells in mice, the agglutination of SA cells and amino acid uptake by SA cells were described. Ricin D and 2 reduced-carboxymethylated chains (RoCM-Ile and RoCM-Ala chains) were prepared as previously described. SA cells were harvested from donor mice 7-8 days after i.p. inoculation, washed with cold Krebs-Ringer phosphate buffer at pH 7.4 and suspended in the same buffer. The 2 constituent chains of ricin D possessed different functions in SA cells. The lethal action of ricin D was due to the inhibition of protein synthesis by the Ile chain. The Ala chain effectively carried the Ile chain on the cell surface, thus altering the conformation of the membrane and accelerating the penetration of the Ile chain into the cells.

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