Replication of Virus Plaques

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Abstract
Summary One of the 2 suspended cell plaque technics described by Cooper(5) has been modified so that its sensitivity and precision for plaque assay of VEE and EEE virus are satisfactory for general purposes. In addition, the modified suspended cell technic permitted nearly quantitative replication of virus plaques. Feasibility of the method in simulated systems for screening of host range or antigenic type recombinants (or mutants) was demonstrated. The excellent technical assistance of Mr. Delbert Davisson in this work is gratefully acknowledged.