BISACK ANALYSIS OF THE PHYTOHAEMAGGLUTININ-INDUCED PROLIFERATION OF HUMAN PERIPHERAL LYMPHOCYTES

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Abstract
The rate of stimulation as well as subsequent cell cycle duration was examined in phytohemagglutinin-stimulated human peripheral lymphocytes grown in vitro in the presence of non-inhibitory concentrations of bromodeoxyuridine. After incorporation of this heavy atom analog of thymidine into replicating cellular DNA, unequivocally metaphase cells which had replicated for 1, 2 and 3 or more cell cycles were identified. Distribution curves for the appearance of metaphase cells in successive generations were analyzed by a computer simulation model and the rate of stimulation (4.5%/h of the remaining unstimulated population) and cell cycle duration (12.3 h) were determined. Autoradiographic results were compared and the possible relationship to the transition probability model for cellular proliferation was discussed.