Monoclonal antibodies specific for simian virus 40 tumor antigens

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Abstract
Thirty hybridomas that secrete Ig against the SV40 tumor antigens were isolated and cloned. Of these, 28 produced antibodies which bound to SV40 large T and 2 produced antibodies which bound to the host 53,000-dalton protein. Large T antigen had at least 1 determinant that it shared with small antigen and had a minimum of 6 unique determinants. Several of the monoclonal antibodies from the L series hybridomas recognized determinants that were present on a subset of the large T antigen from SV40-transformed mouse cells. These monoclonal antibodies should be useful in studies of the structure and function of the SV40 tumor antigens.