PCR cloning and detection of point mutations in the eburicol 14 a -demethylase (CYP51) gene from Erysiphe graminis f. sp. hordei , a "recalcitrant" fungus
- 9 December 1998
- journal article
- research article
- Published by Springer Nature in Current Genetics
- Vol. 34 (5) , 399-403
- https://doi.org/10.1007/s002940050413
Abstract
Molecular studies of some micro-organisms are hampered by the difficulty of obtaining sufficient amounts of nucleic acids. A cloning strategy based on PCR has therefore been used to clone the eburicol 14α-demethylase (CYP51) gene of the obligate fungus Erysiphe graminis f. sp. hordei (Egh) using minute amounts of genomic DNA. The CYP51 gene encodes the enzymatic target of a major group of fungicides. Sequencing CYP51 from different Egh isolates revealed the occurrence of two alleles for this gene. An allele-specific PCR assay was developed to detect each CYP51 allele.Keywords
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