The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase
- 1 July 1998
- journal article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 5 (7) , 585-592
- https://doi.org/10.1038/846
Abstract
Biosynthesis of the corrin ring of vitamin B12 requires the action of six S-adenosyl-L-methionine (AdoMet) dependent transmethylases, closely related in sequence. The first X-ray structure of one of these, cobalt-precorrin-4 transmethylase, CbiF, from Bacillus megaterium has been determined to a resolution of 2.4 A. CbiF contains two alphabeta domains forming a trough in which S-adenosyl-L-homocysteine (AdoHcy) binds. The location of AdoHcy and a number of conserved residues, helps define the precorrin binding site. A second crystal form determined at 3.1 A resolution highlights the flexibility of two loops around this site. CbiF employs a unique mode of AdoHcy binding and represents a new class of transmethylase.Keywords
This publication has 34 references indexed in Scilit:
- CATH – a hierarchic classification of protein domain structuresPublished by Elsevier ,1997
- Refinement of Macromolecular Structures by the Maximum-Likelihood MethodActa Crystallographica Section D-Biological Crystallography, 1997
- [20] Processing of X-ray diffraction data collected in oscillation modePublished by Elsevier ,1997
- The 1.85 Å Structure of Vaccinia Protein VP39: A Bifunctional Enzyme That Participates in the Modification of Both mRNA EndsCell, 1996
- Structure-guided Analysis Reveals Nine Sequence Motifs Conserved among DNA Amino-methyl-transferases, and Suggests a Catalytic Mechanism for these EnzymesJournal of Molecular Biology, 1995
- Universal Catalytic Domain Structure of AdoMet-dependent MethyltransferasesJournal of Molecular Biology, 1995
- Protein Structure Comparison by Alignment of Distance MatricesJournal of Molecular Biology, 1993
- Expression of 9 Salmonella typhimurium enzymes for cobinamide synthesis Identification of the 11‐methyl and 20‐methyl transferases of corrin biosynthesisFEBS Letters, 1992
- Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operonJournal of Molecular Biology, 1990
- The protein data bank: A computer-based archival file for macromolecular structuresJournal of Molecular Biology, 1977