Promotion of Mitochondrial Membrane Complex Assembly by a Proteolytically Inactive Yeast Lon
- 4 October 1996
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 274 (5284) , 103-106
- https://doi.org/10.1126/science.274.5284.103
Abstract
Afg3p and Rca1p are adenosine triphosphate (ATP)-dependent metalloproteases in yeast mitochondria. Cells lacking both proteins exhibit defects in respiration-dependent growth, degradation of mitochondrially synthesized proteins, and assembly of inner-membrane complexes. Defects in growth and protein assembly, but not in degradation, were suppressed by overproduction of yeast mitochondrial Lon, an ATP-dependent serine protease. Suppression by Lon was enhanced by inactivation of the proteolytic site and was prevented by mutation of the ATP-binding site. It is suggested that the mitochondrial proteases Lon, Afg3p, and Rca1p can also serve a chaperone-like function in the assembly of mitochondrial protein complexes.Keywords
This publication has 31 references indexed in Scilit:
- MBA1 encodes a mitochondrial membrane‐associated protein required for biogenesis of the respiratory chainFEBS Letters, 1996
- The Saccharomyces cerevisiae OXA1 gene is required for the correct assembly of cytochrome c oxidase and oligomycin‐sensitive ATP synthaseFEBS Letters, 1996
- Afg3p, a mitochondrial ATP‐dependent metalloprotease, is involved in degradation of mitochondrially‐encoded Cox1, Cox3, Cob, Su6, Su8 and Su9 subunits of the inner membrane complexes III, IV and VFEBS Letters, 1996
- Mutations in RCA1 and AFG3 inhibit F1‐ATPase assembly in Saccharomyces cerevisiaeFEBS Letters, 1995
- Disassembly of the Mu transposase tetramer by the ClpX chaperone.Genes & Development, 1995
- Yta10p is required for the ATP‐dependent degradation of polypeptides in the inner membrane of mitochondriaFEBS Letters, 1994
- Yta10p, a member of a novel ATPase family in yeast, is essential for mitochondrial functionFEBS Letters, 1994
- OXA1, a Saccharomyces cerevisiae Nuclear Gene whose Sequence is Conserved form Prokaryotes to Eukaryotes Controls Cytochrome Oxidase BiogenesisJournal of Molecular Biology, 1994
- Cloning and disruption of the gene encoding yeast mitochondrial chaperonin 10, the homolog of E. coli groESFEBS Letters, 1993
- Site‐directed mutagenesis of La proteaseFEBS Letters, 1991