A Fluorescent Intercalator Displacement Assay for Establishing DNA Binding Selectivity and Affinity
- 18 November 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Accounts of Chemical Research
- Vol. 37 (1) , 61-69
- https://doi.org/10.1021/ar030113y
Abstract
A summary of the qualitative and quantitative elements of a fluorescent intercalator displacement (FID) assay useful for establishing the DNA binding selectivity, affinity, stoichiometry, and binding site size and distinguishing modes of DNA binding is provided.Keywords
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