CCK2 (CCKB/gastrin) receptor mediates rapid protein kinase D (PKD) activation through a protein kinase C‐dependent pathway
- 26 January 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 489 (1) , 101-106
- https://doi.org/10.1016/s0014-5793(01)02076-2
Abstract
Addition of gastrin or cholecystokinin octapeptide (CCK-8) to cultures of Rat-1 cells stably transfected with the CCK2 (CCKB/gastrin) receptor induced protein kinase D (PKD) activation that was detectable within 1 min and reached a maximum (∼10-fold) after 2.5 min of hormonal stimulation. Half-maximal PKD activation for both CCK-8 and gastrin was achieved at 10 nM. Treatment with various concentrations of the selective PKC inhibitors Ro 31-8220 or GF-I potently blocked PKD activation induced by subsequent addition of CCK-8 in a concentration-dependent fashion. Our results indicate that PKC-dependent PKD activation is a novel early event in the action of gastrin and CCK-8 via CCK2 receptors.Keywords
This publication has 43 references indexed in Scilit:
- Activation of Protein Kinase D by Signaling through the α Subunit of the Heterotrimeric G Protein GqPublished by Elsevier ,2000
- An invasion-related complex of cortactin, paxillin and PKCμ associates with invadopodia at sites of extracellular matrix degradationOncogene, 1999
- Gβγ-Mediated Regulation of Golgi Organization Is through the Direct Activation of Protein Kinase DCell, 1999
- The Pleckstrin Homology Domain of Protein Kinase D Interacts Preferentially with the η Isoform of Protein Kinase CJournal of Biological Chemistry, 1999
- Bryostatin 1 Induces Biphasic Activation of Protein Kinase D in Intact CellsPublished by Elsevier ,1997
- Bombesin and neuromedin B stimulate the activation of p42mapk and p74raf-1 via a protein kinase C-independent pathway in Rat-1 cellsOncogene, 1997
- Protein kinase C mu is located at the Golgi compartment.The Journal of cell biology, 1996
- PD 098059 Is a Specific Inhibitor of the Activation of Mitogen-activated Protein Kinase Kinase in Vitro and in VivoJournal of Biological Chemistry, 1995
- Expression and Characterization of PKD, a Phorbol Ester and Diacylglycerol-stimulated Serine Protein KinaseJournal of Biological Chemistry, 1995
- Gastrin and Gastric Enterochromaffin-like Cell Carcinoids in the RatDigestion, 1990