Vanillin Content in Boiled Peanuts

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Abstract
A high-performance liquid chromatographic (HPLC) method for determination of vanillin in boiled peanuts has been developed. Vanillin was extracted with acetonitrile by blending at high speed followed by purification of an aliquot of the extract on a minicolumn packed with Al2O3. Vanillin was quantitated by HPLC on silica gel with n-hexane/2-propanol/water/acetic acid (2100/540/37/2, v/v) as a mobile phase. The recovery of vanillin added to fresh peanut hulls at 0.50 and 2.50 μg/g was 78.7 ± 2.7 and 79.9 ± 3.1%, respectively. The detection limit of vanillin in boiled peanuts was estimated at 0.05 μg/g. UV-detector response to vanillin was linear to at least 2.5 μg/injection. Free vanillin has been found in two commercial brands of boiled peanuts at low ppm levels. Both the kernels and the hulls contained vanillin, which was formed during hydrolysis of lignin, one of the major constituents of the peanut hulls. Since vanillin has a low flavor threshold, it could be considered as one of the major ingredients that determines the flavor of boiled peanuts. Keywords: Vanillin; peanuts; groundnuts; Arachis hypogaea L.; flavor; HPLC analysis; lignin

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