THE NUTRITIONAL REQUIREMENTS OF HEMOPHILUS PARAINFLUENZAE 7901

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Abstract
A simplified synthetic medium has been developed which supports growth of H. parainfluenzae 7901. Arginine, isoleucine, valine, tyrosine, glutamic acid, cystine, leucine, phenylalanine, lysine, and aspartic acid are essential for max. growth of this organism. Biotin, Ca pantothenate, thiamine, and vitamin B6 are essential vitamins. Coenzyme I (or n) and putrescine (or spermine or spermidine) are also essential. A suitable pyrimidine is essential for growth;.uracil, uridine, uridylic acid, cytidine, cytidylic acid, or desoxycytidine can serve in this role. Cytosine is inactive. Glucose and Na acetate are also required for max. growth. Growth of H. parainfluenzae in the synthetic medium is severely inhibited by the single addition of either adenine or guanine. Such inhibition of growth is alleviated by the simultaneous addition of hypoxanthine, or of appropriate combinations of other purine bases.

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