The Axenic Cultivation of Two Reptilian Parasites, Entamoeba terrapinae Sanders and Cleveland, 1930, and Entamoeba invadens Rodhain. 1934

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Abstract
The amoebae were cultivated axenically in a liquid medium free of gross particulate matter. Monox-enic amoeba-Trypanosoma cruzi cultures served as source material. Axenic clones of E. invadens were established by microisolating single trophozoites in microcultures and after a period of incubation transferring the progeny to macrocultures. Addition of 0.059( agar to the medium was required for initiation of all axenic cultures but this ingredient could be dispensed with after several subcultures.